CHO cells are the preferred host expression system for the commercial-scale production of complex biopharmaceuticals (antibodies, enzymes, growth factors, etc.). The aneuploid, proline-requiring CHO-K1 line is a stable subclone of the parental CHO cell line derived from the ovary of an adult Chinese hamster (Puck et al, 1958).
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2 Nov 2004 Under Biosafety Level 3 conditions, eight pools of five human lung cDNA- transduced CHO cell clones were challenged with infectious 15 Dec 2003 Containment level needed to sufficiently protect against harm to the environment. . 31 estimated that a single molecule of Diphtheria Toxin is sufficient to kill a cell). Biosafety Gastroenterit s (Cho era Cho Additional detail about BSL-2 practices and containment can be found in Section IV of Biosafety in. Microbiological and Biomedical Laboratories, or in Appendix G- Cell lines which do not contain known human or animal pathogens are designated BSL – 1. The following list contains human or primate cells that are to be See next page for Biosafety Level 2 cell cultures.
Microorganisms and Cell Cultures GmbH. ADR: Inhoffenstraße 7B 38124 Braunschweig GERMANY. Look at the map. PHONE: +49 (0)531 2616-0. FAX: +49 (0)531 2616-418. EMAIL: Biosafety Level 4 (BSL-4 molecules and cells, organisms and viruses containing such molecules to ensure an optimal protection of public health and of the environment. EU biosafety regulatory framework The assessment of biological risks associated with the use of GMOs and/or pathogens is supported by a comprehensive regulatory framework, in particular at European Union (EU) level.
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Material Safety Data Sheet for Cell Cultures (Biosafety Level 1) 1. Product Identification Name of cell line: CHO (CLS order no. 603479) Designation: Chinese Hamster Ovary cell line, permanent cell line 2.
Mammalian cell lines (Hela, HEK293, CHO Working with infected or transformed cells of laboratory WHO / PHAC etc established biosafety or containment levels or
American Type Culture Collection. Emergency Telephone: Product Testing for CHO-Derived.
Introduction Lentiviruses, a genus of the Retroviridae family, are enveloped retroviruses that replicate in a host cell through reverse transcription. Lentiviruses are classified as Biosafety Level 2 (BSL-2) organisms due to their ability to infect primary human cells.
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EMAIL: Biosafety Level 4 (BSL-4 molecules and cells, organisms and viruses containing such molecules to ensure an optimal protection of public health and of the environment. EU biosafety regulatory framework The assessment of biological risks associated with the use of GMOs and/or pathogens is supported by a comprehensive regulatory framework, in particular at European Union (EU) level. The EU legislation forms the basis of the implementation of biosafety in Belgium. used for mammalian cell gene transfer in the absence of replication within the transduced cells. For baculovirus 168 mediated gene transfer into mammalian cells, the term transduction is used rather than infection due to the inherent inability of the virus to replicate in mammal, ian cells.
3 All laboratories at Anschutz Medical Campus are designed at Biosafety Level 2 containment, at a minimum, with unni-directional inward airlflow (negative pressure
Cells stored for more than 1 day at −80°C quickly lose viability. If properly banked, cells are guaranteed for six months in liquid nitrogen. Storage Buffer: Recovery™ Cell Culture Freezing Medium (Invitrogen #12648-010). Biosafety Level: 2 Instrument Settings: The GeneBLAzer® ADRA1A CHO-K1 DA Cells requires a fluorescence
Human embryonic kidney 293 cells, also often referred to as HEK 293, HEK-293, 293 cells, or less precisely as HEK cells, are a specific cell line originally derived from human embryonic kidney cells grown in tissue culture taken from a female fetus.
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Biosafety level 2 practices and containment facilities for all activities involving HEK-293 cell lines . Required PPE Procedures that may generate droplets (aerosols, splashes, sprays) with HEK-293 cell lines and their derivative should be handled in a Class II BSC with BSL-2 PPE (lab coat and gloves) (9) .
The CHO-S cell line is a stable aneuploid cell line established from the ovary of an adult Chinese hamster (Puck, 1958). The cell line has been distinguished as a separate sub-clone from the The biosafety level for this cell line is 2 where for CHO is only 1.
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Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH
Product Name: Various Cell Cultures at Biosafety Level 1 or 2 COMPANY INFORMATION: CORIELL INSTITUTE for MEDICAL RESEARCH 403 HADDON AVENUE . CAMDEN, NJ 08103 of Applied Biosafety, the topic of biosafety require, ments for "human cell lines" popped up on the BiO' safty Discussion List. Itseems that we biosafety pro fessionals have not done a very good job, in some instances, ofexplaining the differences between BiO' safety Level 1 and Biosafety Level 2 practices to our The CHOZN® Platform, developed by SAFC, is a CHO (Chinese Hamster Ovary) based cell expression system designed for accelerated selection and scale up of clones producing high levels of recombinant protein. Key to the CHOZN® Platform is the development of the CHOZN® ZFN (Zinc Finger Nuclease) modified cell lines (GS -/- and As with other mammalian cell lines, when working with CHO DG44 Cells (cGMP banked), handle them as potentially biohazardous material under at least Biosafety Level 1 containment. Note that the CHO DG44 Cells (cGMP banked) are provided in freezing medium containing DMSO, and the components of the product may be absorbed into the containment – Biosafety Level 3, and maximum containment – Biosafety Level 4. Biosafety level designations are based on a composite of the design features, construction, containment facilities, equipment, practices and operational procedures required … As with other mammalian cell lines, when working with CHO DG44 Cells (cGMP banked), handle them as potentially biohazardous material under at least Biosafety Level 1 containment.